The statistics of the background regions of interest (ROIs) are shown in Figure 2, and we see that the image has a homogeneneous background (on average 103.5 counts/pixel with a standard deviation of 1.1 between the rows).
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For contrast-to-noise ratio calculation, myocardial and background regions of interest (white) were drawn on a midventricular short-axis image.
In addition, it provides several different procedures for analysis, such as the computation of means or percentages of any background variable of interest for a whole country or subgroup within a population (IEA 2012).
Conversely, in some instances, the larger background region of interest used in the Southampton method can add benefit by reducing noise and therefore uncertainty in the measurement of non-specific uptake [17].
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We developed an ImageJ macro to perform post-processing of the ratio images from the raw 340 nm- and 380 nm- images, assuring that the background region of interest was never occupied by a unwanted fluorescent cell.
Categories for the other background variables of interest are shown in Table 1.
The SNR was calculated as the mean signal intensity within the affected region (MaR or infarcted myocardium) divided by the standard deviation of signal intensities within a background region of interest.
