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The infection site areas of a resistant sib from the same M2 family were similar to wild type confirming that the observed phenotype of 2F was largely due to the mutation found within the Lr34 gene and unlikely to be the result of accumulated background mutations (data not shown).
Other wild-type and SBEIIa mutant segregants had normal stature and flowering phenotypes under both greenhouse and field growth conditions, and these lines were chosen for further propagation and backcrossing to the parental variety to remove background mutations (data not shown).
Similar(58)
We failed to detect chromosome III fragmentation in a sml1Δ WT RAD53 background carrying the pif1-m2, rrm3Δ, or pif1-m2 rrmutationsions (data not shown).
The Sanger sequencing confirmed all the mutations (data not shown).
The sequences showed no mutations (data not shown).
However, the temperature-sensitivity is less penetrant in the BY4741 background than in the W303 background (data not shown).
All cis2 promoter-less control vectors showed similar background activity (data not shown).
The sense probes did not detect signals stronger than the background signal (data not shown).
As previously demonstrated, all controls exhibited only background staining (data not shown) [ 16].
The binding of Olig1 to the LIF promoter was around the background level (data not shown).
Control experiments with no primary antibodies showed only a faint background staining (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com