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In each group of rats, only cells on both sides that exhibited significant levels of DAB reaction product in their nucleus greater than that of tissue background levels were counted.
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Levels above a distinct background level were counted as scattering events.
Those cells that contain signals intense enough to be distinguished from background level were counted as positive cells.
An arbitrary level of fluorescence was determined as the background standard and wings above or equal to this level were counted.
The recombinants that were in a white (clear) background and non recombinants that were in a blue background were counted.
Radioactive hot nodes on the basis of counts more than 10-fold above background level were defined as SLNs [ 11, 12].
White tumor nodules against a blue lung background were counted.
In each tissue section, only granule density (the number of granules over the counted area) exceeding three times background level was defined as positive hybridization signal.
No significant difference in background level was observed.
The number of pixels within a given region of interest (ROI) were counted at the single-cell level and normalized against the background staining.
6) Counting: plasma samples were counted using appropriate standards and blanks for background in a well counter.
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