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The T686A mutant showed background levels of kinase activity (Fig. 3A, 3B), confirming that this mutant is kinase-dead [19].
As shown in Fig. 4A, Chk1D469G immunoprecipitated from untreated cells has essentially background levels of kinase activity, approximately the level of signal observed when the "kinase dead" Chk1D155A protein is assayed.
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A non-smoker group provided background levels of BoE.
Only background levels of staining were observed (data not shown).
Background levels of genetic alterations.
Only background levels of 3-nitroTyr were detected with dONOO.
Background levels of empty clones were less than 10%.
This can be the background level of translational selection.
Western blotting was performed to determine levels of kinase activation.
Transcriptional activity levels of kinases were estimated by taking advantage of the genome-wide data coverage of GeneSapiens to determine the overall body-wide background levels for the transcriptional activity of each kinase gene.
Since the only differences between these animals are the Met mutations and the background, this study illustrates that either the kinase mutational structure itself or the level of kinase they impose (or both) influence the tissue-specificity for tumor formation.
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