Sentence examples for background levels of gene from inspiring English sources

Herein, we present an automated high-throughput screening method that identifies synthetic riboswitches that display extremely low background levels of gene expression in the absence of the desired ligand and robust increases in expression in its presence.

In the present study, the differences in background levels of gene expression between unexposed m PPARα and h PPARα mice must be duly noted, along with differences in TRI-induced changes in m PPARα mice observed between the present study and past studies (Ramdhan et al. 2008) and discrepancies between mRNA and protein expression for some genes.

In further improvements of the system, tetR was placed under the control of tetO elements to create an autoregulatory loop, thus avoiding problems caused by toxicity of constitutive tetR expression and reducing background levels of the gene of interest in the repressed state [3], [4].

The GAL4-VP16 syntranscriptionription factor is driven by the Mitochondrial Ribosomal Protein 7 (MRP7) constitutive promoter, resulting in very low background levels of reporter gene expression.

We found that we could only amplify similar background levels of plastidial genes for Col-0, KO and OEX plants confirming the lack of specific interaction between AtWhy2 and plastidial DNA in vivo (data not shown).

In order to assess the significance of the conservation of specific gene pairings across related yeast species, we investigated the background levels of small-scale gene pair synteny across four species.

This was done to match the gene expression experiments to the conditions used to measure flowering time and examine if the high background levels of flowering time gene expression in the -DEX plants was also observed in plants growing in these non- sterile conditions.

We also include 0.5 mM 3-aminotriazole to reduce a background level of His gene expression.

While background level of reporter gene activation in the two cell types was less than 1.5-fold, crosslinking the surface protein with a polyclonal antibody resulted in a 10-fold increase in NFκB-dependent luciferase expression in tetherin-expressing cells and led to the concomitant phosphorylation of IKKα/β and degradation of IκB.

Furthermore, genes within ±1 Mb of the Fendrr locus were not significantly differentially expressed any more than background levels of local enrichment, suggesting that the Fendrr gene does not act as a local enhancer.

The atp6 and atp8 genes were characterized by background levels of replacement mutations, relative to synonymous mutations.