Sentence examples for background levels of fluorescence from inspiring English sources

The cells only displayed background levels of fluorescence when stained with corresponding isotype controls (Figure 1J-L).

Only in the presence of sCD4 was weak binding of gp120 to the CXCR4-proteoliposomes detected above the background levels of fluorescence observed in untreated cells or cells incubated only with sCD4 (Figure 3A).

Isotype control antibodies were used to correct for the background levels of fluorescence.

Background levels of fluorescence were determined by labelling cells with secondary antibody only.

In contrast, low nonspecific binding was observed from VEGFR negative 4T1 breast cancer cells, as evidenced by background levels of fluorescence signal in all the groups.

For each quantification and treatment, 10 blanks (animals not exposed to Nile red) were used to account for background levels of fluorescence.

The negative control was undifferentiated cells and was used to set the background level of fluorescence.

This phenomenon increases the effective background level of fluorescence, which has to be taken into account when interpreting the loading results.

For Kv4.2 fluorescence, the background level of fluorescence at the cell periphery in the absence of co-expression of KChIP1 (typically 10% of the total [ 12]) was determined for each experiment and subtracted from values for cells expressing KChIP1.

As internalized molecules cannot be removed by cell washing or filtration, these cells contribute to the background level of fluorescence; however, they do not compromise our analysis of protein internalization.

To determine the background level of fluorescence, using the Cluster 3.0 program, the expression data was log2 transformed, mean centered, and normalized across the time course for each gene.