Natural heat shock promoters constitute a simple inducible misexpression system, the main disadvantage is a high background level of expression.
In order to determine the background level of expression, we investigated the number of mRNAseq reads mapping to intergenic regions.
Likewise, in the endometrium tamoxifen, but not raloxifene, may have oestogenic-like effects due to recruitment or coactivators to a subset of genes, and this aspect may vary in different tissues depending on the background level of expression of coactivators such as SRC-1 [ 21].
The misassigned reads were spread over approximately 2500 genes on average; i.e., they contribute to a low background level of expression, although genes for nuclear proteins tended to have more such reads than other genes (Additional file 7: Table S6A).
The larger cluster (labeled green in Figure 5E, F, G, I) of cells displays strong GFP expression while the second cluster (blue) displays low to background levels of GFP expression.
Herein, we present an automated high-throughput screening method that identifies synthetic riboswitches that display extremely low background levels of gene expression in the absence of the desired ligand and robust increases in expression in its presence.
In contrast, the magnitude of [18F]FEDL binding pancreatic tissue was significantly lower in the regions remote from the site of tumor growth (red rectangle Fig. 4B, shown magnified in 4D), which was consistent with low (background levels of HIP/PAP expression in these remote regions of pancreas (Fig. 4G).
Background levels of SOC1 expression in the -DEX TG plants were high as they were similar to Col plants at ZT15.
In the present study, the differences in background levels of gene expression between unexposed m PPARα and h PPARα mice must be duly noted, along with differences in TRI-induced changes in m PPARα mice observed between the present study and past studies (Ramdhan et al. 2008) and discrepancies between mRNA and protein expression for some genes.
The GAL4-VP16 syntranscriptionription factor is driven by the Mitochondrial Ribosomal Protein 7 (MRP7) constitutive promoter, resulting in very low background levels of reporter gene expression.
