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Neutral Red blanks were also included to remove background levels of dye absorbed by the inserts.
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A non-smoker group provided background levels of BoE.
Only background levels of staining were observed (data not shown).
Background levels of genetic alterations.
Levels of dye inside of neurons were detected by confocal microscopy (Olympus).
In these data, background was not subtracted, thus we had to estimate a background level for each dye swap to get information about the hybridization level.
fLow or undetectable levels of dye-CMC and pNPG activity.
Co-hybridization of cDNAs from each T. cruzi life-cycle stage with a reference cDNA sample comprised of all four life-cycle stages on oligonucleotide, whole genome microarrays, revealed that over 83% of the oligonucleotides detected transcript above background levels and were consistent between dye-swap replicates in at least three life-cycle stages (10,256/12,288).
The DNA yield and level of dye incorporation were measured using the ND-1000 Spectrophotometer.
The level of dye incorporation was evaluated using a spectrophotometer (Nanodrop ND1000, LabTech).
Ct values of -RT controls did not exceed background levels.
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