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Dephosphorylated MvSrc1 and MvSrc2 showed background levels of activity, while the ATP-treated enzymes were active.
Further deletion of the promoter region was expected to abolish the promoter activity, and accordingly, cells containing the construct plhr29-lacZ, which lacks the −35 box, displayed background levels of activity (Figure 1B).
All 12.1-Ala control constructs showed background levels of activity, confirming the peptide-specific p53 induction.
Surprisingly, however, pMITF-MCAT exhibited near background levels of activity in DTC1, Kao, MST-2 and MST-3 cells, indicating that endogenous EWS/ATF1 is unable to activate a transiently introduced Mitf-M promoter in CCS cells.
For example, the somatostatin promoter (Δ(−71 SomCAT) is highly active (260% of RSVCAT) in DTC1 cells while exhibiting only background levels of activity (0.03% of RSVCAT) in Jeg3 cells.
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By nature it is sensitive to robust changes in the energy content of the regional activation that are sufficiently high to stand out from the background level of activity.
But the new findings indicate that people with the short version may have a chronically higher background level of activity, says Canli, whose team reports its findings online this week in Proceedings of the National Academy of Sciences.
Constructs containing the amino-terminus of Teashirt3 (i.e., full-length Teashirt3, and the N-term and ZF3 fragments) showed significantly higher HDAC activity, whereas those without the amino-terminus showed background level of activity.
Although our model is generic and is not intended to accurately describe a hepatocyte per se, it indicates that positive interactions with a background level of activity of the cAMP pathway is another possible explanation of these observations.
To put these values into context, the background level of activity associated with the metal-depleted enzyme was ca. 1% of wild-type activity.
Sensitive detection of the response under study often depends not only on the product Φrelε(λirr) but also on the background level of activity of the caged compound prior to irradiation.
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background levels of fluorescence
background levels of oil
background levels of cell
background levels of Cd
background levels of methylation
background levels of lead
background levels of air
background levels of staining
background levels of endotoxin
background levels of centriole
background levels of T-cell
background levels of replacement
background levels of disease
background levels of radiation
background levels of blood
background levels of bioluminescence
background levels of dye
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