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Mouse immunization with Ad5 vector resulted in background levels of T-cell activation (0.83%).
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The NA peptide pool (N1/CA PP), used as a control in this experiment, induced only background levels of T cells, demonstrating that little or no T-cells induced by the infection with the swine virus were present in the lungs three days after challenge.
Low levels of T-cell reactivity to pools 71 80 and 81 86 of the gag peptides (10× and 5× background, respectively, and >25 sPBLs/10 PBLs) and env V3 and IDR peptides (9× and 6× background, respectively) were observed with unfractionated PBLs.
The calmer men also had much higher levels of T-cells, which fight the AIDS virus.
We show that T arrays quantitatively monitor the expansion of T-cell clones after viral infection with high sensitivity (1 in 106 cells), and with sufficient resolution to identify single clones in a background of polyclonal peripheral blood T-cells.
The background response of T cells isolated from mice vaccinated with pVax-luc was less than 0.1% (Fig. 3a).
Background levels of fluorescence were determined by labelling cells with secondary antibody only.
In contrast, the normal human cells only demonstrated negligible background levels of NF- κB activity.
Histologically, IBM shows chronic myopathic changes in a background of T cell-rich endomysial inflammation [ 1- 3].
In cells overexpressing STIL-ΔCC only background levels of centriole amplification could be detected.
(A – C ) Wild type cells that were in S16/G216 display background levels of γH2Av staining.
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