Sentence examples for background level was set from inspiring English sources
Therefore the background level was set to a log2 intensity level of 9.0 for the E. coli arrays, which is a slightly higher level than the intensities of the custom negative control probes (Figure 4 and 5).
The background level was set to be the same in all calculated difference maps by applying the same absolute plotting parameters as used for the control difference maps.
In the probeset selection procedure, the background level was set to 3.1 on the basis of the mean signal intensities of 62 reporter probesets (i.e. several controls that are not in the investigated poplar genomes; mean value 2.5 and mean maximum value 3.5).
GAPDH was used as a negative control and background levels were set to the largest observed GAPDH value.
The background fluorescence level was set with the negative controls, and images were analysed using Zen image analysis software 2009 (Zeiss).
A threshold level was set by eliminating all pixels with intensity lower than 99.0% of the pixels of the background ROI.
