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Cells with fluorescence intensities above background levels were scored as expressing αSMA.
Staining intensities above background levels were scored as positive.
Cells in which the dsRED signal did not exceed background levels were scored as 'miRNA positive'.
No significant difference in background level was observed.
The background level was defined as the level at which low level transcription becomes indistinguishable from other background signals.
However, for other matrices, like those representing the TATA-box, the background level is prohibitively high when the score threshold is reduced.
Signals less than 10 were set to 10 to eliminate the inaccuracy of analyzing genes expressed at near background levels from being scored as differentially expressed.
Therefore spots near the background level have a higher QM if the background level is higher.
Sections were considered positive when ≥ 1% of the tumor cells had membranous staining above the background level and the intensity of EGFR reactivity was scored as +1, +2 and +3.
Suppression of TPE or PEV was scored in the w− background by comparing expression levels of the reporter genes in a mutant background to expression levels in their siblings, who received a balancer chromosome rather than the chromosome bearing the mutation of interest.
The latter nodule was scored as below-background on OSEM, and background on BPL.
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