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No significant difference in background level was observed.
In the presence of the enzyme alone no oxidation of this dialkylhydrazine above the background level was observed.
Importantly, Ad5-CRAD replication in only 1% of the cells resulted in a ∼50% decrease of CAR intensity in all cells, and a further decrease close to the background level was observed when 10% of the cells were infected (Figures 2A and 2C).
A significant increase in mutation yield over the background level was observed at fiber concentrations > 1 μg/cm (p < 0.005).
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When the OsASNase2 sense probe was used, only weak signals as background level were observed (Fig. 3d).
Nevertheless, a decline to background levels was observed approximately three weeks immediately after the television campaign [ 16].
Using the filters described previously, no fluorescence above background levels was observed in either the acceptor or the donor channel.
When the 12.1 peptide was expressed as a free peptide, no activity over background levels was observed, while CI2-constrained 12.1 peptide activated p53 in approximately 25% of the transduced population.
However, only background levels are observed when the reporter construct is inserted into euchromatin where the w+ gene is fully expressed.
Even higher background levels were observed at ZT11.
As a control for non-specific binding, Q-PCR was conducted to detect transgene transcript levels in wild type cDNAs but only background levels were observed.
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