Highest sensitivity and cleanest background was obtained with overnight 37°C incubations followed by high-stringency washes (see below).
In some papers, background model is obtained with averaging several consecutive frames and Gaussian distribution for background points is considered.
Upon completion, the gel was stained in Coomassie Blue staining solution until a clear background was obtained for scanning with GS-800 calibrated densitometer (Bio-Rad, USA).
A notable recovery of native genetic background was obtained by minimising coancestry with the exogenous individuals calculated from information on marker genotypes.
Tlr9 −/− mice with C57BL/6 background were obtained from Bioindustry Division Oriental Yeast Co. , Ltd
Tlr3 −/−, Tlr4 −/−, Tlr5 −/−, IL-1R1 −/− and MyD88 −/− mice with C57BL/6 background were obtained from The Jackson Laboratory.
Cbs+/− mice, on a C57BL/6 background were obtained by mating male Cbs+/− mice with female wild-type C57BL/6 (Cbs+/+) mice.
PP1γ2 transgenic positive mice in a PP1γ −/− background were obtained by first crossing transgenic founder mice with PP1γ −/− CD1 female mice or PP1γ +/− male mice, then intercrossing PP1γ2 transgenic/PP1γ+/− progeny.
No signal above background was obtained when the primary antibodies were replaced with pre-immune sera.
TSK/+ and homozygous pallid (pa/pa) mutant mice with a C57BL/6 background were obtained from the Jackson Laboratory (Bar Harbor, ME, USA).
