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Background-noise influence was removed by subtracting the background intensity from the captured images.
For each spot and in each channel (Cy3 and Cy5), True et al. substracted the median background intensity from the median foreground intensity, and subsequently the log ratios of cancer expression to benign expression were computed.
Fluorescent spot signals were obtained by subtracting background intensity from the total spot intensity.
ArrayVision software read the slides and normalized the data by subtracting background intensity from the spot intensity values.
min estimates the mean squared deviation of the logarithm of the estimated background intensity from the actual background value.
The intensity of each sample and standard was adjusted by subtracting background intensity from the measured intensity.
Similar(51)
The median of signal and background intensities from the Alexa Fluor 555 and 647 channels were quantified using GenePix Pro 6.0 image acquisition and analysis software (Axon Instruments, Union City, CA, USA).
Here, median spot and background intensities from the red (Cy5) and green (Cy3) channels were analysed.
It is customary to subtract background intensities from the spot intensity, and this also produces missing values.
Raw intensities were adjusted by subtracting the background intensity from each probe, and the data were then summarised by taking the median intensity across the 16 replicates of each probe.
In all these instances, the mean background intensity from an area outside of the cells was subtracted for each pixel.
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