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Scaling was adjusted such the low background intensity is visible.
The part of zero order frequency is background intensity.
The results showed that the appropriate threshold is slightly below the local background intensity.
Background intensity was subtracted from each reading.
The background intensity is fixed to 2500 intensity levels.
The average background intensity did not differ between genotypes.
Background intensity was determined as described above for determining data to include in MAK2 model-fitting.
Only cells with a cytoplasmic FI greater than 10% of background intensity were considered.
Deconvolution also flattened contours caused by uneven background intensity while preserving PSF-like objects.
Hence the background intensity distribution of the homogeneous coated Fn was obtained.
Background intensity computed from a set of negative controls was subtracted from each analytical data point.
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