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The homozygous kcbp-1 lines, which were identified by detecting the T-DNA insertion (please see the Author response image 2), were used to be the null KCBP background in our complementation tests, and the primers were already listed in the primer list in the previous version.
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The T474D mutant was not included in our complementation assay because we could not recover T474D expressing transformed lines.
Sex-specific effects on TAG storage were also observed in our initial complementation tests with different mutant alleles of dSdc.
In our experiments, complementation of the virus is limited to occurring solely in cholinergic neurons, which express the rabies-G induced by the ChAT-Cre transgene.
In our hands, complementation of sk156 with SPL15m leads to increased levels of SPL15 transcripts and decreased levels of miR156b transcripts.
Critically, in our trans-complementation assays, CFP-WT PML co-expression restores efficient basal or arsenic-enhanced sumoylation of HA-PMLF52/54E HA-PMLF52/54Eat of PMLL73E, mirroring Nbuteformationeverg. 5b and Supplementhat Figure 7b).
A 33-bp deletion in the sor1 mutant gene compromises the gravitropic response; the results of our complementation analysis indicate that the gravitropic response can be restored by a functional SOR1 gene.
Our complementation assays reveal that engineered mutations within, or proximal to, the catalytic triad of the N-terminal glutamine amidotransferase (GATase) domain inactivate the complementation activity of T. gondii CPSII and demonstrate a critical dependence on the apicomplexan CPSII GATase domain in vivo.
Indeed, our complementation experiment (Fig. 5) showed that other factors encoded at the clpK locus are of importance in resistance toward thermal stress.
Our complementation experiments indicate that EspL is a newly identified ESX-1 component involved in ESAT-6 secretion and stable EspE expression.
Background knowledge 2. In our study we also consider a different background knowledge.
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