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The background image was set to many randomly located white dots.
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Background images are set to have the same size as object images.
The background value for each image was set using the "threshold" function of the program to a level that would exclude all pixels in control tissues stained with secondary antibody alone.
The background fluorescence of GFP images was set to the fluorescence intensity of < 20 using Image J software.
During this process, the flame portion of the image is set to white (binary value 1) and the background is set to black (binary 0).
Thereafter, gray-level maximum z-projection images were set to eliminate background based on intensity threshold.
Thresholds of confocal images were set in Adobe Photoshop to exclude background staining.
In each image, the background value was set using the "threshold" function of the software to exclude all pixels surrounding the positive stained signals.
The background image is from the Hubble Space Telescope.
Background image is from MODIS mosaic of Antarctica (MOA 58.
The background threshold was set the same for each image analyzed.
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