Sentence examples for background fluorescence was used from inspiring English sources
The resulting background fluorescence was used to subtract the positive signals from using a specific primary antibody staining for the cell imaging (using the Image-Pro Plus software).
To define gates, Fluorescence Minus One (FMO) controls were used and background fluorescence was excluded using secondary antibodies alone.
LightCycler analysis software was used for quantifications, and background fluorescence was removed using the noise band.
Background fluorescence was measured using unlabeled cells and cells labeled with isotype control or secondary antibody alone; and used to set gating parameters between positive and negative cell populations.
The collected spectra were averaged and the background fluorescence was subtracted using an asymmetric least squares smoothing.
Background fluorescence was determined using isotype-matched Ig.
Background fluorescence was measured using the 3xSLv1 probes.
Background fluorescence was assessed using appropriate isotype antibodies.
Background fluorescence was measured using cells stained with secondary antibody only.
The assay was repeated in triplicate and the background fluorescence was estimated using a control zinc incubation with MBP for which no precipitate was observed.
Crossing points, defined as the point at which the fluorescence rises above the background fluorescence was determined using the "Fit Point Method" in the LightCycler software 3.5.3 (Roche Diagnostics).
