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Background fluorescence was determined using isotype-matched Ig.
Background fluorescence was determined from wells containing probes without cells and subtracted from respective red and green fluorescence values.
Background fluorescence was determined by fluorescence from DMEM alone and subtracted from all experimental values.
At each reading, background fluorescence was determined with pure n-butanol and subtracted from each value.
Background fluorescence was determined in wells containing assay buffer and substrate without cell lysate.
The background fluorescence was determined by using E. coli JM109 cultures without plasmid.
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Integrated fluorescence intensities relative to background fluorescence were determined by ImageJ program.
Irrespective of how background fluorescence is determined by a particular investigator, it must be subtracted from that of the raw fluorescence.
For each tissue, background GFP fluorescence was determined by FACS analysis of cell suspensions from wild type FvB mice (Charles River), and dead cells were eliminated by high propidium iodide fluorescence.
Fluorescence was determined before (background) and immediately after diffusion of the test compound into a single astrocyte, and the areas labelled by the probes were determined with MetaVue software.
An arbitrary level of fluorescence was determined as the background standard and wings above or equal to this level were counted.
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