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We analyzed in wild type and hog1 background, expressions of two genes, GPD1 and GPP2, involved in glycerol synthesis and two general stress response genes CTT1 and HSP12, predominantly controlled through STRE.
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(A ) qPCR quantification of splice isoforms produced by ectopically expressed mrps16 relative to background expression of each isoform.
To remove non-expressed genes, we applied filtering criteria and established a background expression cutoff on values summarized by the PLIER algorithm (see Methods).
Expression data showing upregulation in A172 glioblastoma cells expressing I κB α-SR served as control to identify background expression of non-NF κB-regulated genes.
A convenient zero background expression cloning strategy was developed.
All of the promoters exhibited low background expression under normal conditions and could be strongly induced by Cd stress.
Lower sensitivity of cRNA based Luc reporter constructs was due to its background expression, 2-fold lower expression, and around 4 h delay in expression of luciferase.
Lastly, we investigated a method to inhibit background expression through competitive inhibition by supplying the reaction with 5′ cap structure analog.
In combination with rtTA, tTS actively suppresses background expression or "leakiness" without impeding the inducibility of the target gene, providing a true "On/Off" transgenic switch.
The location and orientation of the components was optimized in order to obtain a low background expression combined with high inducibility.
A molecular approach was conducted to optimize hsp22 promoter element in order to decrease the background expression level of the reporting gene and to increase the sensitivity of the bioassay for testing endocrine disruptors.
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