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Likely because constitutive expression of rtTA is toxic and also leads to higher background expression of the regulated gene.
Figure 2A B shows typical snapshots of the population that allowed us to quantify the amount of fluorescence, the background expression of the promoter and the proportion of dead cells.
First, it helps to eliminate unwanted selection during the vector maintenance due to the low background expression of the protein.
This study demonstrated the feasibility to further improve tetracycline responsive promoters, especially for reduction of background expression of the Tet regulatory system in the off-state.
Although addition of ey-GAL80 greatly reduces background expression of the c316-GAL4 inserthese these flies were less healthy than other lines and thus were only used for functional imaging, but not generally for behavior experiments.
M23 has a 164 kb deleted region containing the fatty acid desaturase gene FAD2-1A[ 66, 67] and therefore, as expected, we detected little more than background expression of the FAD2-1A gene in M23.
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This discrepancy probably resulted from the low background expression of these genes and/or the insufficient transcriptome sequencing depth.
More information on the background expression of biomarkers in the general population is needed (i.e. what is the normal range?).
To clarify these questions, we studied apoptosis in Cx-transfected HeLa cells with no background expression of Cx in the wild-type state.
Thus, the high background expression of AKRs may set the obstacle to distinguishing small differences in AKRs between tumor and adjacent tissues with IHC.
As evident from the flow cytograms of DMSO-treated control cultures, the background expression of histone γH2AX differed markedly among the four tested cell lines.
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