Sentence examples for background dye from inspiring English sources
Over 60% of the cells were PKH67-positivePKH67-positiveincubation with H. pofygyrus vesincubationared with 1.5% wH.n incubated with background dye (Supolygyrusry Fig. 5).
We assessed data quality with the minfi R package [ 16] and normalized intensities using the ARRm software, which corrects for probe type, background, dye, and position effects [ 17].
The dead spermatozoa show positive red staining with eosin and the live spermatozoa not staining, show a white color in contrast with the nigrosin background dye.
Top, diagram of dye loading protocol, showing the methods for assessing background dye loading under control conditions (no additional CO2), dye loading during a hypercapnic challenge (PCO2 55 mmHg) and the use of the hemichannel blockers to probe the identity of the hemichannel required for CO2 sensitive dye loading.
All ethidium bromide fluorescence values were corrected for background (extracellular) dye fluorescence and expressed as a percentage relative to the value obtained following complete permeabilization of the cells with 50 μM digitonin.
Many problems that plague microarrays (inaccuracies in background correction, dye-gene effects, skew toward one channel, dye crosstalk, and contaminating fluorescence) cannot be accurately assessed in data from filter-based microarray scanners due to this limitation and this can lead to erroneous data [ 9, 17].
Images were then processed using the Feature Extraction software (v 9.1, Agilent Technologies, Palo Alto, CA) generating the net signal for each channel (Cy3 and Cy5) and p-values after background and dye-bias correction.
Background subtraction and dye normalization for each array was performed within the Agilent Feature Extraction software with default settings.
Arrays were scanned with Agilent's G2565AA Microarray Scanner System and analyzed using Agilent Feature extraction software (Agilent Technologies), which calculates log ratios and P values for valid features on each array and provides a confidence measure of gene differential expression by performing outlier removal, background subtraction, and dye normalization for each feature.
Median of foreground signal intensity subtracted by background for each dye channel was used for analysis.
