Although enzymes for both amylose and amylopectin syntheses had higher activity in IR36, amylopectin synthesis was seemingly more important for accelerating SAR because an elevation of amylose synthesis ability alone in the T65 genetic background did not result in the same level of SAR as IR36.
Unlike the ICR strain, we observed that global inactivation of GSK-3α on the C57BL/6/129 background did not result in improved glucose/insulin tolerance or enhanced hepatic glycogen accumulation (Figure 7) or insulin sensitivity (data not shown).
However our observations argue against this hypothesis (at least in the context of GT1b), as introducing the NS5B gene derived from DEB025res replicon into a WT background did not result in transfer of resistance.
Deletion of IRC10 in the ade2 -min3 background did not result in a blebbing phenotype, but an ade2 -min3 end3 Δ mutant blebbed and was temperature sensitive at 37°.
By contrast, control depletion of the splicing factors in the rrf-1 background did not result in significant reporter derepression in the mitotic region.
Although silencing of only ALS3 in the NIL- Ol-1 background did not result in increased susceptibility to On, this does not exclude the possibility that ALS3 is involved in resistance.
Removal of Fj, and therefore Ds phosphorylation, from the phosphomutant background did not result in a significant change in stable amount at the junctions confirming that the observed reduction in stability of the phosphomutant was primarily due to a loss of Fj phosphorylation of Ft. A bar chart showing the stable amounts of Act- ft-EGFP in each condition is provided in Figure 4F.
Despite the competent PsLAR activity in vitro, expression of PsLAR driven by the Arabidopsis ANR promoter in wild-type and anr knock-out Arabidopsis backgrounds did not result in PA synthesis.
In summary, although it cannot be completely ruled out that the effect of blocking starch synthesis is dependent on the genetic background, current evidence on mutants and complementants in cw15 background shows that blocking starch synthesis does not result in over-accumulation of oil in Chlamydomonas cells.
An angular resolution of ∼3 4° is recommended both for better detection significance and improved false alarm rate, considering that finer angular resolution does not result in improved background rejection when the coded aperture method is used.
