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For background determinations, values of non-stimulated controls were subtracted from the different stimuli of each individual donor.
A pGEX4t3-tag plasmid was included to express tagged GST alone, which is necessary for serologic background determinations.
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Neighbouring ROIs as well as their spill-out regions are excluded from background determination.
By comparing with background determination in 2007, there was no significance for ∑7PBDEs.
Moreover, neighbouring ROIs (if present) as well as their spill-out regions are excluded from background determination.
The presented approach to partial volume correction using local background determination distinctly improves quantitative accuracy of the correction in comparison to similar, previously described model-free approaches relying on a homogeneous background for the whole lesion.
Each plate contained 12 wells of uninfected cells for background determination.
The negative control probes are intended to have no hybridization and these are used by feature extraction software for background determination.
Background Determination of the role of steroid hormones in expression and regulation of endometrial glucose transport 4 (GLUT4) in humans is important for understanding endometrial disorders such as polycystic ovary syndrome (PCOS), a common hormone-imbalance disease.
Each participating center was asked to test in triplicate 18 preselected donors (5 in the first phase, 8 in the second phase and 5 in the third phase) with two synthetic peptides HLA-A*0201 HLA-A*0201d epitopes of CMV and Influenza) as well as PBMCs in medium alone forestrictednd depitopestiof.
Background: The determination of the pleural fluid (PF) carcinoembryonic antigen (CEA) concentration has proved helpful in the differentiation between pleural effusions (PE) of malignant and benign origin.
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