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The background density was set to 0 g/cm3.
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The spectral densities are set to.
A background ROI was set adjacent to the left ventricle.
The background threshold was set at twice the background fluorescent signal plus 3 standard deviations.
The background correction was set to: offset = 50.
Background white noise was set at 70 dB.
After the background staining correction, the optical density was calibrated and the area of interest was set through: hue, 0 30; saturation, 0 255; intensity, 0 230.
For collagen signal density calculations, a noise removal filter with kernel size of 3 × 3 was used to define the boundary between foreground and background, and the lower threshold in the histogram was set to mean voxel intensity value.
The threshold was set with a dark background.
His lineup picture was set apart by a different background.
Background gates were set at 0.05%, and positive results were defined as >2-fold >2-foldund.
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