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Background data are shown in table 2 and more detailed information about the total sample is described elsewhere.
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Relative intensities of the protein bands were quantified by scanning densitometry using the NIH Image Software (Image J, http://rsb.info.nih.gov/ij/), and each value background-corrected. Data are shown as arbitrary units and are the average ± SEM of 4 5 animals/group.
The expression patterns were generally consistent across the three strains and data are shown for the CD1 background.
Representative sample data are shown.
Background subtraction is not suitable for these microarrays and in preliminary analysis normalisation of background-corrected data was shown to decrease the data quality as compared to normalisation without background correction.
An example of the effect of subtraction of a uniform background on Y patient data is shown in Fig. 6.
The scatter-plot of background-subtracted mean values (normalized data) is shown in Figure 6B.
The raw data is shown as dots in the background, the data filtered with the Savitzki Golay filter shown as a continuous line.
Representative data were shown.
Data is shown as neuromast/background intensity.
Further cross tabulations between patients' demographic and clinical status and deprivation category indicated that there were no significant differences between patients of different social background (data are not shown and is available from the corresponding author).
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