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For further analysis, genes with signal intensity below 10 after background correction were excluded to avoid taking genes whose alterations are not easily distinguished from noise into subsequent analyses.
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Raw data analysis, signal quantification and background correction were performed with Arrayvision version 6.0 (Imaging Research, Ontario, Canada).
Bias correction and background correction were performed through ProteinPilot.
Normalization and background correction were done by RMA.
Background corrections were then applied to exclude array elements with intensities less than the average intensities of control elements (designed against non- Drosophila DNA) in both channels for downstream analysis.
Background correction was made using a reference blank KBr pellet.
No background correction was performed.
Background correction was done for autofluorescence.
A robust Edwards background correction was applied.
No background correction was performed before normalization.
Background correction was performed by subtracting the median global background from the median local background from the signal intensity.
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