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The background correction was set to: offset = 50.
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Threshold and background corrections were set based on Red-Green scattergram, generating a co-localization image (co-localized pixels in white) and pixels distribution scatter plot (co-localized yellow pixels along the diagonal).
Beam hardening correction was set to 60%%.
Therefore, we use the HCSSM to compare the ability to find differentially expressed genes when no background correction is used versus a non negative background correction method [ 21].
Prebackground adjustment was set to adjust for GC Content and probe sequence and RMA background correction was performed.
The algorithm of Fig. 6, right was first tested on the test set without the source, to prove that the background correction was reliable.
Background correction was performed using the subtract background command with the rolling ball radius set to 50 pixels.
Background correction was made using a reference blank KBr pellet.
No background correction was performed.
Background correction was done for autofluorescence.
A robust Edwards background correction was applied.
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