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Formazan formation was determined by absorbance at 590 nm, and the background correction was measured at 690 nm.
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Local background correction was performed by moving the mask to measure fluorescence at a nearby region, and this value was subtracted from the ciliary Smo fluorescence.
Bias Correction and Background Correction was checked for protein quantification and normalization.
Background correction was done using the normexp method by Ritchie et al.62.62
Background correction was made using a reference blank KBr pellet.
No background correction was performed.
Background correction was done for autofluorescence.
A robust Edwards background correction was applied.
No background correction was performed before normalization.
Background correction was performed by subtracting the median global background from the median local background from the signal intensity.
For example, after a background correction was applied to data from a microarray experiment, further background correction is neither possible nor useful.
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