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Background correction was determined to be unnecessary, as all tissue for each gene was run through the ISH procedure, including phosphor-screen exposure and scanning, and there were no observed differences in white matter signal intensities between groups.
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The background correction was set to: offset = 50.
Formazan formation was determined by absorbance at 590 nm, and the background correction was measured at 690 nm.
After determining the fluorescence intensities of both channels, a background correction was made by subtracting the local background value from the foreground intensity.
Bias Correction and Background Correction was checked for protein quantification and normalization.
Background correction was done using the normexp method by Ritchie et al.62.62
Background correction was made using a reference blank KBr pellet.
No background correction was performed.
Background correction was done for autofluorescence.
A robust Edwards background correction was applied.
No background correction was performed before normalization.
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