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Both background correction and normalisation were done with RMA (Robust Multichip Average) algorithm (Irizarry et al, 2003).
Background correction and normalisation were performed in the R computing environment (Ihaka and Gentleman, 1996) using the robust multi-array average algorithm.
Single dye labeling of samples, hybridization procedures, data acquisition, background correction and normalisation were performed at the PartnerChip facilities following the standard protocol defined by NimbleGen [ 103, 104].
Background correction and normalisation of the dataset have been performed using the Affy package of Bioconductor (RMA algorithm) in the statistical environment, R (version 2.10.1).
Expression data from 36 OVCA cell lines were subjected to background correction and normalisation using the Robust Multichip Average algorithm in the Affymetrix Expression Console (http://www.affymetrix.com).
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The Affymetrix signal extractions showed a much larger heterogeneity than the background corrections and normalisations used on the other platforms.
We used RMA and Bioconductor in R [70] for background correction and normalization [71], [72].
Arrays were normalized using RMA background correction and quantile normalisation in R v.2.15 (Additional file 3).
Background correction and quantile normalisation via the dasen method were conducted individually for the two applied chemistries (Infinium I and II) as well as for the intensities of methylation (m) and un-methylation (u).
Currently Guide can also perform background correction and quantile normalisation [ 18] automatically for Illumina Mouse WG-6 v2.0 array, thus making it more convenient for the user by requiring only the raw data as input.
More details on sample preparation is given in previous descriptions of this experiment [ 29] and the data is available in GEO [ 45] with the accession number GSE53170 [ 46]. Background correction and quantile normalisation was performed on the microarray data (GSE53170) using the R package LIMMA [ 47] from Bioconductor [ 48].
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