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The ChIP signal in a promoter region of Il2ra was used as a background control.
Freshly prepared medium was analyzed aside the samples as background control.
Background control ChIP was performed using intergenic regions without H3K27me3 mark to determine the ChIP specificity.
Cells that did not express GLUT5 were used as background control.
Sonicated chromatin was precipitated with anti-H3K27Ac, anti-BRD3, anti-BRD4 or IgG (negative background control) antibodies.
After substrate of background control, cell viability was expressed as a ratio of absorbance relative to that of control.
Background control wells (i.e., GST wells instead of GST-3MST, n = 16) were also prepared for all plates.
Background control (Bkg) sections were prepared without the primary antibodies.
The sixth probe used as a constitutive background control (contig 12730c) gave a consistent signal.
All experiments were done in triplicate, and T cells alone were used as the background control.
Unmodified TPGS-b- PCL-ran-PGA) NPs were also analyzed as a background conTPGS-b- PCL-ran-PGA
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