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The working range was 5 320 nM with negligible possible interference from the different background composition of water samples by optimization of the binding conditions.
It is based on analysing the response of a chromatographic column, equilibrated with a constant feed, to a small impulse perturbation of this background composition.
To determine the effects of pseudo-backcrossing on genomic background composition, nine elite pseudo-BIL (BC3F2) families were selected for genome scanning using 61 SSRs, 35 and 26 of which were located on six carrier and six non-carrier chromosomes, respectively (Additional file 1).
In principle, the method can be extended to co-adsorption of several components, by using several injections of different mixtures, on the same background composition, and measuring both retention times and areas of the set of peaks produced by each experiment.
The entropy of an alignment site is a measure of variation in its state and a criterion by which sequence conservation can be objectively quantified: entropy is zero at invariant sites and has a maximum value of one when all possible states (residues) occur equally frequently (or proportional to the background composition).
The codons GAC (Asp), GGC (Gly) and GCC (Ala) also revealed biases that mirrored the background composition.
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The procedure is then repeated for a number of discrete background compositions defining composition paths.
By using the same footage we were able to select control clips with virtually identical viewing angles, postures, background compositions, lighting, etc.
A modified version, Nc', has been developed which also account for the background nucleotide composition of the gene; this is advantageous in situations where the composition varies among the genes analyzed [ 75].
Separation parameters such as background buffer composition, voltage, temperature, and nebulizer pressure were optimized using a multivariate central composite design (CCD).
Richard Eyre is a theater director who has been responsible for many brilliant Shakespeare productions; and his eye for setting, background and composition is superb.
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