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Background binding was determined from the absorbance generated in wells with blocking solution alone.
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Based on the ratio of 'light' to 'heavy' isotopes for each protein, enrichment through specific binding to LINKIN over background non-specific binding was determined.
Specific binding was determined by subtracting the background absorbance from the absorbance in experimental wells.
Within the context of a CWR22Pc-R1-AD1 CWR22Pc-R1-AD1 CWR22Pc-R1-AD1ively expresses a strucellally full-length AR containing a H874Y mutation23, the rank order of antagonist backgrounds determined as ARN-509 > Hydroxyflutamide > Enzalutamide > Nilutamide > Bicalutamide (Fig. 3c).
Nonspecific binding was determined in the presence of 1 μM spiperone.
Nonspecific binding was determined in the presence of 5 (1 μM) as a competitor.
Nonspecific binding was determined with 5 μM -vesamicol.
Peptide binding was determined using ELISA.
Specific binding was determined by subtracting values of non-specific binding from that of total binding.
Background binding was corrected using reactions containing no peptide.
Background binding was subtracted from the value obtained for binding to the consensus DNA sequence.
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