Sentence examples for background binding of the from inspiring English sources

Since cyclin B1 is not expressed for much of G1, this provided a near perfect control for background binding of the GNS1 antibody.

It should be noted that, after subtraction of the background binding of the negative control protein (Trx), the binding affinity of the peptide P1 to the human antisera after vaccination increased only about one fold compared with the antisera before vaccination (Fig. 6, panel D).

The background binding of the control antibody IIB6 scFv phage increased when using very high scFv phage particle concentrations.

ELISA was done as described in Section 2.5.1 Background binding of the serum to the plate was corrected for by subtracting the average binding signal of antibody to MA from that registered for the hexane coated wells.

The negative controls consisted of incubation of fixed protoplasts with the Alexaflour488-conjugated anti-rabbit secondary antibody alone, which produced no signal, indicating that the immunolocalizations of anti-AtCNGC5 and AtCNGC10 to the plasma membrane resulted from primary antibody localization and not background binding of the secondary antibody.

The background binding of the PET tracer to both the noninflamed knee joint and periarticular bone (marrow) (in both knees and other physiological locations) could lead to underestimation of targeting properties of the applied PET tracer since PET imaging of a target relies on the contrast between the target and its background.

In G0 cells, the only antibody that gave significant enrichment on the lamin B2 origin DNA was the one against Rb (∼4-fold over background); binding of Rb to the origin remained constant at all subsequent time points.

Our studies determined that with low DNA input, the standard MBD-cap protocol results in high levels of background binding of DNA to the MBD-linked beads, and thus only minor enrichment of methylated sequences, whereas with standard DNA inputs, background binding is proportionately significantly decreased and methylation enrichment reaches at least 7-fold.

Using this modified MBD-cap protocol, background binding of DNA to the MBD-linked beads was minimal even at low DNA inputs, with methylation enrichment reaching 30-fold.

Here, a two state reaction model was used to compensate for background binding of Bni5 to the antibody-coated chip surface.

However, showing the limitations of this method and obviously due to a nonspecific background binding of FAM-FFCK, most of the 108 proteins turned out to be non-proteases.