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This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime.
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Raw, mixed signal images were analysed by the Maestro 2.2 software to isolate the autofluorescence (based on the control animal) and QD (or Alexa 680) fluorescence.
The ethnic background was assigned based on self-report questionnaires.
All digital images were background-subtracted based on conjugate control.
The number of cells, percentage of damaged cell membranes or oxidatively stressed cells, and autofluorescence data were retrieved based on autofluorescence of Chla (670 nm), PI-labelled cells (585 nm) and ROS Green (533 nm) (Additional file 1: Figure S1).
Thus, in vivo quantification of lens transmission is based on autofluorescence of lens proteins [ 31- 33].
Many groups have reported the development of methods for diagnostic classification of oral tissue based on autofluorescence and/or diffuse reflectance spectroscopy.
In the case of the stroma, it was possible to differentiate between normal and dysplastic tissue based on autofluorescence changes but not via analysis of SHG signals.
The in vivo diagnosis of these small-size lesions is currently based on autofluorescence bronchoscopy with targeted biopsies of the areas that display less fluorescence.
There were only two studies based on Asian background and one based on African background.
We thresholded images based on background fluorescence and measured total length of objects above background.
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