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Rather, it seemed to me, the background area was done in aquatint, a related process using a porous resin resulting in a uniform, granulated effect.
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The signal intensity in the background area is usually low.
Background correction was done for autofluorescence.
Background staining was done with propidium iodide (Invitrogen).
Background staining was done with propidium iodide (Invitrogen, Taastrup, Denmark).
Background correction was done by subtracting local background median density from signal median density.
Local background correction was done at scanning using default values.
The background staining was done with Mayer's haematoxylin.
I'm sure the background check and everything was done".
Descriptive analysis on background characteristics of respondents was done.
All image analysis was done after background subtraction.
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