Sentence examples for background and specificity from inspiring English sources

Third, because biotin is a biological molecule, endogenous biotin can cause background and specificity problems in biotin-rich tissues and extracts.

We previously developed a reversion assay with six different base substitutions in an essential codon of the Saccharomyces cerevisiae TRP5 gene, yielding a mutagenesis assay with extremely low background and specificity for point mutations (Williams et al. 2005).

While advances in photolithography and solid phase oligonucleotide synthesis technologies improved the amount of sequence that could be analyzed with such arrays, this strategy was plagued by relatively high background and low specificity, as it was highly sensitive to sequence context.

We optimized methods to reduce background and maximize the specificity of staining.

In addition to its simplicity, a further advantage of gel electrophoresis, whether performing band-shift assays like here or SDS gels for western blots, is that it provides a direct visual control of the background and of the specificity of the signal.

In summary, we have developed an assay to quantify specific gene expression changes in a complex background with high sensitivity and specificity.

However, some background information about sensitivity and specificity is needed in both cases.

Thus, there is an unmet clinical and experimental need for SOX11-targeting assays with low background, high specificity and robust performance in multiple applications, including immunohistochemistry (IHC-P) and flow cytometry, which until now has been lacking.

This feature might contribute for lower background, higher specificity and the possibility of using smaller samples.

This method was used to remove signal background (a negative enrichment strategy) and, thus, enhance sequence specific quantitation and specificity.

The sensitivity (100 ng) and specificity (high-background staining) of Coomassie may remain a problem despite the improved colloidal version; however, it is cost-effective, relatively simple to use and offers good compatibility with MS [ 11, 80- 84].