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Recorded fluorescence was corrected for background and normalized to the baseline value (∆F/F0).
(c) The width of a single coincidence peak shows that the fluorescence lifetime of the LH2 complexes close to the antennas (sum) is strongly reduced compared with an ensemble of LH2 complexes in solution (corrected for the background and normalized to the maximum).
Density for TrkB and BDNF was corrected for background and normalized to the density of the corresponding band for GAPDH.
Surface STIM1 images were thresholded to a level 2X background and normalized to a total STIM1-GFP signal thresholded at 1.5X background.
Curves were corrected for both non-specific photobleaching (NSPB) that occurred during imaging and background, and normalized to the first image in the series.
Each immunoblot was measured relative to the background and normalized to the endogenous controls (β-tubulin).
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Intensities from nuclear and cytoplasmic masks were background corrected and normalized to the intensity at the beginning of the experiment.
Intensities are background corrected and normalized to incident laser power.
The recorded absorbance spectra were background corrected and normalized to the absorbance at 310 eV according to Christl and Kretzschmar [25].
For FRAP analysis of MannII-GFP, mean intensity values of fluorescence recovery were background subtracted and normalized to the pre-bleach intensity value of the Golgi and plotted as a percent of this value over time.
Signals were background subtracted and normalized to the unstimulated control.
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