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Dancers could be seen in the background, adjusting their costumes before the mirror as they prepared to come on, and at one point Harrell, in the half-dark, carrying an iPad, walked from the audience and into the crossover/dressing space.
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Genes with average background adjusted fluorescent intensity value >50 across all arrays were retained in the analysis.
Using Illumina's Genome Studio, background adjusted β-values and assay control probe information was used for initial quality control.
For each sample, we evaluated a minimum of one thousand CFP/YFP positive cells that fell within the background adjusted gate (Fig. 1a, panel 2).
Arrays were quantified on the basis of spot intensity relative to background, adjusted for experimental variation between arrays using average intensity over multiple channels, and fitted to a previously described error model [29] to determine significance (type I error).
Expression levels were background adjusted, quantile normalised and log2 transformed.
The values are background adjusted, normalized, and log transformed.
The raw intensities were background adjusted by the Subtract method.
The raw intensities were background adjusted, log2-transformed and RMA-normalized with RMAexpress.
Microarrays were background adjusted, normalised, summarised and log transformed according to GC Robust Miroarray method.
Perfect- match values were background adjusted, normalized using invariant set normalization and log transformed.
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