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Genes with average background adjusted fluorescent intensity value >50 across all arrays were retained in the analysis.
Using Illumina's Genome Studio, background adjusted β-values and assay control probe information was used for initial quality control.
For each sample, we evaluated a minimum of one thousand CFP/YFP positive cells that fell within the background adjusted gate (Fig. 1a, panel 2).
Arrays were quantified on the basis of spot intensity relative to background, adjusted for experimental variation between arrays using average intensity over multiple channels, and fitted to a previously described error model [29] to determine significance (type I error).
Expression levels were background adjusted, quantile normalised and log2 transformed.
The values are background adjusted, normalized, and log transformed.
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Dancers could be seen in the background, adjusting their costumes before the mirror as they prepared to come on, and at one point Harrell, in the half-dark, carrying an iPad, walked from the audience and into the crossover/dressing space.
The RMAExpress V1.05 software package (http://rmaexpress.bmbolstad.com) [ 16] was used to generate probe level intensities by setting the operating parameters as: Background adjust: Yes; Normalization: Quantile; Summarization method: Probe level model.
Robust multi-array average (RMA) procedure was applied to the entire set of raw signals (i.e..CEL files) in order to background adjust and normalize microarray intensities and to generate gene expression values.
The robust multiarray average (RMA) algorithm as implemented in the Bioconductor package 'affy' (Gautier et al., 2004) for R was used to background adjust and normalize the raw data files for each dataset that showed upregulation of Sirt3 and gene ontology-related gene sets upon DR (except for GSE24504, for which the GEO series matrix was used).
It suggested the difference might be because those from richer backgrounds adjusted more easily to changes in school meals, or because the less privileged students were more represented among those getting lowest scores, and improvements were harder to achieve for those at the bottom than in the middle.
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CEO of Professional Science Editing for Scientists @ prosciediting.com