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Non-specific background adhesion was determined by measuring binding to Duffy antigen coated on Petri dishes.
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The percentage of adhesion was determined by dividing the corrected (background subtracted) fluorescence of adherent cells by the total corrected fluorescence of cells added to each microplate well.
Coating adhesion was determined by measuring delamination during Rockwell testing.
Cell retention and adhesion was determined using phase contrast microscopy under laminar flow.
Cell adhesion was determined relative to total cells plated.
Tendon adhesion was determined histopathologically and biomechanical test was performed.
Monocyte-endothelial cell adhesion was determined using fluorescence-labeled monocytes.
Moreover, the effect of RGZ on cell adhesion was determined.
We now have demonstrated both in vitro and in vivo evidence for the importance of RBC signaling and have also shown that SS RBC adhesion is determined by genetic polymorphisms in the signaling pathway that activates adhesion.
The quality of the induced adhesions was determined according to the following scores: 0 (no adhesion), 1 (avascular adhesion), 2 (filmy vascular adhesion), and 3 (dense, vascular adhesion).
Changes in actin cytoskeletal organization and focal adhesions were determined by fluorescence staining using FITC-phalloidin and anti-vinculin antibody/rhodamine-conjugated secondary antibody, respectively.
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