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However, the allelic ga20ox1-3 mutant did not show differences from its background accession Col-0 (Fig. 5A).
Also, the double mutant ga20ox1 ga20ox2 showed a higher LDMC growth percentage compared with its background accession Col-0 (150·1 ± 57·8 versus 114·8 ± 6·4 %).
Seeds of this mutant and its corresponding background accession Ws were obtained from the library present at Wageningen UR Plant Breeding [ 22].
Among the induced mutants, ga5 showed a higher growth percentage compared with its background accession L er for the trait SFW (Fig. 5A).
Seeds of brl3-2 and brl3-3 mutands and their corresponding background accession Columbia-0 (Col-0) were kindly provided by Prof. S.C. de Vries, Laboratory of Biochemistry Wageningen University [ 26].
These findings were supported by PCA using all evaluated traits, in which ga20ox1-3, gandox2-1 and ga20ox1 ga20ox2 tended to cluster near their background accession Col while the ga5 mutant was further from its wild-type genetic background, L er (Supplementary Data Fig. S2).
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The double mutant ga20ox1 ga20ox2 showed the lowest performance among the Col background accessions.
Evaluation of sugarcane germplasm with DNA markers helps understand the extent of genetic diversity among parental lines and the difference in genetic background among accessions.
The biographies are organized not chronologically but by topics: the emperor's family background, career before accession, public actions, private life, appearance, personality, and death.
The double mutant lacking both C6-aldehydes and jasmonates (aos-hpl) is an engineered T-DNA insertion line in AOS resulting in generation of aos loss-of-function plants in the trichomeless background (gl-1, accession Col-0) [25].
Both mutant lines were backcrossed four times to the Bay-0 wild type to homogenize the accession background to Bay-0.
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