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The release of reducing sugars from CSCB was determined with dinitrosalicylic acid, because detection with bicinchoninic acid resulted in very high background absorbances (data not shown).
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However, the temperature-sensitivity is less penetrant in the BY4741 background than in the W303 background (data not shown).
The sense probes did not detect signals stronger than the background signal (data not shown).
Similar results were obtained from data corrected for isotype control background (data not shown).
Background absorbance not associated with the absorbance peak at 260 nm (i.e., Fe(II) dissolved from pyrite or increasing absorbance at lower wavelengths due to RNA) has been subtracted from all of the curves.
The background absorbance values were not higher than 0.2 AU.
No interfering background staining was observed (data not shown).
A similar but less consistent pattern was observed for PM10 and PM2.5 absorbance with NO2 (data not shown).
The collected fractions, which showed peak absorbance at 280 nm (data not shown) were immunoprobed.
However, no clear protein peak was eluted with maltose as judged from UV absorbance at 280 nm (data not shown).
Only background levels of staining were observed (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com