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The background absorbance was determined using samples incubated in the presence of HRP-conjugated anti-rabbit IgG alone (without primary antibody) and then subtracted from the measured values.
Background absorbance was determined by incubating media with substrate alone and subtracting the values from wells containing cells.
Similar(58)
Background absorbances were determined and subtracted from the absorbance determined for each individual reaction.
A background-subtracted absorbance was determined by measuring each sample at both 620 nm, the absorbance maximum for Evans blue, and 740 nm, the absorbance minimum.
The absorbance was determined at 410 nm.
Absorbance was determined at 517 nm.
WST-1 absorbance was determined as described previously [37].
OD260nm absorbance was determined with simultaneous 1 ml fraction collection.
The absorbance was determined at 405 nm in triplicates.
The absorbance was determined at 480 nm.
Absorbance was determined at 450 nm colorimetrically.
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