Sentence examples for background absorbance using a from inspiring English sources

The absorbance was measured at wavelengths of 490 nm and 690 nm (background absorbance) using a microplate reader (MRX Revelation, Dynex Technologies).

RNA concentration was determined by 260/280 nm optical absorbance using a NanoDrop 2000 spectrophotometer (Thermo Scientific).

Collagen assay was performed by mixing lung homogenates with Sircol Dye reagent and measuring absorbance using a plate reader.

Spectra were background corrected using a reflective gold slide and converted to absorbance using the Kramers Kronig equation as per standard FTIR analysis method (Roessler 1965).

After 24 h, cell survival was measured using a Premixed WST-1 Cell Proliferation Reagent (Clontech, 630118), and the absorbance of each sample was measured at 450 nm against the background control using a multi-well plate reader.

FPCL media was then collected and aliquoted into a 96-well culture plate for absorbance reading at 450 and 650 nm (background reference λ) using a 96-well BIO-RAD microplate reader.

Draw the background using a pen.

We avoided the background absorbance of plasma by using a diazo-coupling limulus amebocyte lysate (LAL) assay that gives a magenta coloration (detection limit: 1.6 pg/ml) (Associates of Cape Cod, East Falmouth, MA, USA), and by heating plasma at 65°C for 30 minutes to inactivate inhibitors as described [ 8].

Absorbance was measured at 450 nm (subtracting 570 nm background signal) using an UVM340 plate reader with DigiRead software (Asys Hitech, UK).

The background absorbance was determined using samples incubated in the presence of HRP-conjugated anti-rabbit IgG alone (without primary antibody) and then subtracted from the measured values.

Background absorbance was measured using the blank and subtracted from all absorbance measurements.