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SK-Br-3 cells transfected with a pcDNA3.0/Neo vector backbone were selected and maintained in the presence of 500 µg/ml G418.

SK-Br-3 cell clones transfected with a pcDNA3.1/Zeocin vector backbone were selected and maintained in the presence of 200 µg/ml Zeocin.

Two significantly differentially expressed miRNAs and four miRNAs predicted to target genes involved in the terpenoid backbone biosynthesis pathway were selected and validated by quantitative reverse transcription PCR.

To validate the data obtained from high-throughput sequencing, two significantly differentially expressed miRNAs miR156f-3pp and miR157andp) and four miRNAs (miR5021, miR5163, miR5293, and novel_miR_27) predicted to target genes involved in the terpenoid backbone biosynthesis pathway were selected and their expression levels quantified using stem loop qRT-PCR (Fig.  4).

Excision of the pKO3 plasmid backbone was selected for by subsequent plating on LB agar lacking NaCl and supplemented with 6% d-sucrose followed by incubation at 30°C.

From a diverse feature space of a family of amphiphilic macromolecules of linear and aromatic mucic acid backbones modified with varied aliphatic chains and conjugated with differentially branched poly ethylene glycol), a key molecule (carboxyl-terminated, C12-derivatized, linear mucic acid backbone) was selected for its ability to preferentially bind scavenger receptor A (SR-A) as the key target.

The miR-122 backbone was selected for these experiments given that it has been demonstrated to yield homogenous guide strand sequences as opposed to the more heterogeneous products from miR-31 [32].

The G-quadruplex backbone atoms of the whole 300 ns trajectory were selected for calculation and analysis.

Finally, cells having lost the plasmid backbone containing the URA3 cassette were selected by growth on 5-FOA media.

All parchment specimens (dimensions 0.6 × 1.2 cm) were selected from the backbone area, marked with the two ink formulations on the corium face of the parchment according to a scheme described below.

Multiple real network traces were selected from the link between CERNET backbone and Jiangsu CERNET network to evaluate PortView.