Bacteria transformed with FGF-10, FGFR-1, FGFR-2 shRNA in pLKO.1-puro lentiviral backbone were obtained from National RNAi Core Facility (Academia Sinica, Taipei, Taiwan) and scramble control vector was obtained from Addgene.
Exchange rate constants for the more slowly exchanging amides of the protein backbone were obtained from H exchange-in measurements at pH 7.49.
Oligonucleotides (20 bp) with phosphorothioate backbones were obtained from Synthetic Genetics (San Diego, CA, USA) dissolved in 1×Tris-EDTA pH 7.4.
Similar to the polymerizations of these amines with an equimolar diacrylate, novel linear poly amido amine)s containing secondary and tertiary amines in the backbones are obtained from these polymerizations as reflected by 13C NMR (INVGATE).
Backbone resonance assignments were obtained from HNCACB, CBCA CO NH, HN CA CO, HNCO and HNCANH spectra at 600 MHz H frequency, acquired using unmodified Bruker pulse programs and a protein concentration of 400 µM.
The strength deterioration effects are inherently considered in the ASCE 41 backbone curves as these were obtained from the cyclic envelope (PEER/ATC 72-1 2010; LATBSDC 2014), whereas the degradation of stiffness and energy dissipation capacity is modelled explicitly using a peak-oriented degrading hysteresis model based on cumulative energy dissipation in ETABS 2016 (CSI 2016).
N-H backbone assignments for UbcH7 were obtained from the BMRB entry 15498 (Serniwka & Shaw, 2008).
Additional empirical restraints for base pair, phosphodiester backbone, and deoxyribose pseudorotation were obtained from canonical values derived from B-type DNA.
Sequence-specific assignments for the backbone atoms of kurtoxin were obtained from an analysis of heteronuclear 3D NMR spectra [HNCACB, CBCA CO NNH, HNCA, and HN CO CA] recorded in 25% CD3CN at pH 4.0 and 288 and 278 K using a uniformly N- and C-labeled protein.
The yield and ultimate rotations of all specimens were obtained from backbone curves.
The backbone resonance assignments for Sk45 were obtained from the set of triple resonance experiments, HNCO, HN CA CO, HNCA, HNCACB, CBCA CO NH, and HBHA CO NH.
