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The insertion DNA and vector backbone were assembled into DNA multimers by prolonged overlap extension PCR as described elsewhere [ 40].
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These were assembled into a backbone assembly of 26,064 contigs with an average length of 1011 bp and a median length of 815 bp (fully described in [ 24]).
The DNA fragments were assembled into the backbone plasmid vector of pBlueScript II SK, linearized with restriction endonucleases HindIII and PstI using the Gibson assembly cloning kit (New England Biolabs).
The pGEM backbone was excised from pHTK [34] by digesting with Nar I- Not I, and pGEM and the components described above were assembled into the pSTCII-GFP plasmid through several cloning and subcloning steps.
Supervisors were assembled into substitute repair crews.
The passengers were assembled into an area of about.
ESTs were assembled into contigs using CAP3 [70].
These genotypes were assembled into diplotypes.
In total 37,383 reads were assembled into 2,821 contiguously assembled segments.
Images were assembled into plates using CorelDraw software.
Acquired images were assembled into movies using Metamorph™ 5.0.
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